Pcr Cycle Graph. Most tests like the broad institute test used by mit use a 40 cycle protocol. Exponential growth and the detection of this leads to a strong straight curve growth phase in most common amplification plot forms.
3 basic pcr steps include. Each cycle of amplification doubles the number of molecules in a sample. Show the bands you expect to see on the empty gel image.
If the virus isn t detected within 40 amplification cycles the test result is negative.
Explain what the four lanes of your gel would look like. The ct value is essentially the number cycles of doubling. The pcr test uses amplification cycles to find viral rna. Cycle threshold ct in sars cov 2 rt pcr a guide for health protection teams about interpreting ct in real time reverse transcription polymerase chain reaction rt pcr assays performed for sars.
